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We took PST II as the primary
plasmid, which consist of alkaline phosphatase promoter (PhoA promoter), translation enhancer sequence, Shine-Dalgano sequence, ST II sequence, Amp and Tet resistance gene, and replication origin.
用PSTⅡ作为起始质粒，其结构包括碱性磷酸酶启动子（PhoA promoter），翻译增强子序列，Shine-Dalgano序列，STⅡ序列，Amp及Tet抗性基因，复制起点。
A minimum plasmid named pMini was constructed, containing only kanamycin
resistant gene and pUC18 replication origin.
结果　首先构建了质粒pMini,仅含有卡那抗性基因和pUC18复制起点。
Cloning and Study on the Replication Origin of the Integrated F Plasmid in Escherichia colt
大肠杆菌中整合状态的F′质粒复制起点的克隆和分析
The replication origin of the integrated F' plasmid was cloned by means of marker rescue. No difference in incompatibility and acridine orange sensitivity was found between the mini-F plasmid constructed from such an origin and the autonomous F' plasmid.
用标记获救法克隆了整合状态的F′质粒的复制起点,证明了由这一复制起点构成的mini-F质粒在不亲和性和对吖啶橙的敏感性方面和自主状态的F′质粒都没有不同。
Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid. No structural difference was found between them.
对这一复制起点和来自自主状态的F质粒的复制起点进行了亚克隆,并作限制性内切酶酶切分析比较,没有发现两者在结构上有差异。
20% of the integra-tive suppression strains of these mini-F plasmids were found to be recA dependent, irrespective of the origin of replication (F or F' plasmid) and the direction of replication (uni- or bidirectional).
这些质粒的整合抑制菌株中都有约20％是recA依赖的,不管这一mini-F质粒的复制起点来自F或F′质粒,也不管这一质粒在游离状态中的复制方向是单向或双向。
DNA replication in eukaryotes is initiated at discrete sites on chromosome through the coordinated action of a number of replication initiation factors, this process is best understood in yeast Saccharomyces cerevisiae, where a six protein origin recognition complex (ORC) binds to specific DNA sequences near the origin of replication and recruits other replication initiation factors like cdc6, cyclin-dependent kinase 1 (cdk1) and MCM2-7 to form the pre-replication complex (Pre-RC).
ORC最早在酵母菌中发现,由6个亚单位组成,并与特异性DNA复制序列和其它复制启动因子(如cdc6,cdk1和MCM2-7)结合,形成复制前复合物。 最后,复制前复合物与活化的细胞周期蛋白激酶(cdks)、DNA复制延长因子(如cdc45、DNA多聚酶和复制蛋白A)在复制起点上结合,启动DNA复制。
plasmid origin
replication, derived
pACYC177, is compatible with generally used ColE1
derived plasmids. It facilitates the coproduction of protein from these plasmids and that from ColE1
derived plasmids.
表达载体的复制起点来源于pACYC177，用此表达的蛋白可以方便地与通常使用的ColE1来源的表达载体表达的蛋白共表达。
The genome of porcine circoviruses consists of circular and single-stranded DNA molecules,there are many open reading frames(ORFs)in the genome,of which,the two main ORFs encode the Rep protein which is involved with the replication of porcine circovirus and the Cap protein:Rep and Rep′ bind to double-stranded DNA fragments comprising of the origin of replication of PCV,but the binding sites of the two proteins are distinct.
猪圆环病毒是单链环状DNA病毒,其基因组中包含多个阅读框,其中2个主要的阅读框分别编码复制起始蛋白Rep和Rep′以及病毒衣壳蛋白:Rep和Rep′结合由PCV1的部分复制起点组成的双链DNA片段,但这2种蛋白的结合位点有细微差别;
Plasmid pSV1,in E. coli SM10λπ could be replicated and mobilized because of the chrosomomal DNA of E. coli SM10λπ possess in the tra and the π genes that originate from the plasmids,RP4 and R6K,respectively.
带有pSV1质粒的SM10λπ寄主菌，由于SM10λπ染色体上带有RP4的诱动性基因和促进R6K复制起点启动复制的π基因，故pSV1质粒能够复制且可被诱动。
mini-F plasmids with ISI sequence and origen of replication from F and F' plasmid have been constructed.
构建带有IS1的mini-F质粒,它们的复制起点分别来自F或F′质粒。
Analysing the sequence of the plasmid pBL29 tested ,we found that the molecular weight of the plasmid pBL29 is 3711bp,that it's many unique restriction endonucleases,Km resistance genes and abundant A/T base pairs of replicating site are on the plasmid pBL29.
对质粒pBL29进行测序和分析,证明了质粒pBL29大小为3711 bp,具有大量的单酶切位点、编码卡那霉素抗性基因以及富含A/T序列的复制起点。
the authors describe the construction of a Escherichia coli-Bacillus subtilis shuttle vector pUSL16 which were d
erived from plasmids pUC18 and pREP9. This shuttle vector can be used to clone g
ene in Escherichia coli and Bacillus subtilis.
作者用DNA重组技术，构建了大肠杆菌－枯草杆菌穿梭质粒载体pSUGV4，它是以大肠杆菌（Escherichia　coli）质粒载体pUC18为骨架，与来自穿梭质粒表达载体pREP9含有革兰氏阳性菌复制起点（ori＋）和卡那霉素抗性（kanr）基因片段重组而成．上述穿梭质粒载体可用于在大肠杆菌（E．coli）和枯草杆菌（Bacillus　subtilis）中进行基因克隆工作．
The characterastics of the expression vector are as follows: (1), contains SV_(40) early promotor and origin of DNA
该载体具有以下结构特点：(1)，含SV40早期启动子及DNA复制起点；
查询“复制起点”译词为用户自定义的双语例句&&&&我想查看译文中含有：的双语例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& The mitochondrial DNA (mtDNA) of goose liver (from single animal) is a circular DNA molecule with 16.45x103 base pairs. The restriction endonuclease cleavage map of this mtDNA, using six different restriction enzymes, is reported here. The sites are as follows: 1 for SalI, BgIII; 2 for BamHI; 3 for EcoRI; 4 for BglI and 5 for HindIII. The fragments corresponding to 16 cleavage sites were ordered and precisely located. The position of D-loop and the direction of replication of the mtDNA were also determined. &&&&&&&&&&&&采用从单只鹅肝脏分离纯化的线粒体DNA(mtDNA)建立了6种限制性内切酶16个切点的酶切图谱。这6种酶在鹅肝mtDNA上的切点数目分别为:SalⅠ和BglⅡ各一个,BamH Ⅰ、EcoR Ⅰ、Bgl Ⅰ和Hind Ⅲ分别为2、3、4和5个。此外,还确定了鹅肝mtDNA的复制起点与方向。&&&&&&&& The replication origin of the integrated F' plasmid was cloned by means of marker rescue. No difference in incompatibility and acridine orange sensitivity was found between the mini-F plasmid constructed from such an origin and the autonomous F' plasmid. Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid. No structural difference was found between them. These results suggest that the differ... &&&&&&&&&&&&用标记获救法克隆了整合状态的F′质粒的复制起点,证明了由这一复制起点构成的mini-F质粒在不亲和性和对吖啶橙的敏感性方面和自主状态的F′质粒都没有不同。对这一复制起点和来自自主状态的F质粒的复制起点进行了亚克隆,并作限制性内切酶酶切分析比较,没有发现两者在结构上有差异。本文的结果提示,F质粒和F′质粒在发动染色体复制中对recA基因的依赖性的不同,可能与质粒整合在染色体上的位置不同有关。&&&&&&&& We have reported the dependence of recA gene for the replication of chromosome of E. colt initiated by the F' plasmid but not the F plasmid. mini-F plasmids with ISI sequence and origen of replication from F and F' plasmid have been constructed. 20% of the integra-tive suppression strains of these mini-F plasmids were found to be recA dependent, irrespective of the origin of replication (F or F' plasmid) and the direction of replication (uni- or bidirectional). The reported experimental results tend to sugg... &&&&&&&&&&&&我们曾报道整合的F′质粒所发动的大肠杆菌染色体复制依赖于recA基因,而整合的F质粒则不。构建带有IS1的mini-F质粒,它们的复制起点分别来自F或F′质粒。这些质粒的整合抑制菌株中都有约20％是recA依赖的,不管这一mini-F质粒的复制起点来自F或F′质粒,也不管这一质粒在游离状态中的复制方向是单向或双向。实验结果说明,质粒的整合位置是决定由整合质粒所发动的染色体复制对recA基因的依赖性的主要因素。&nbsp&&&&&&&&相关查询:
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