Recombinant Human Apolipoprotein E (ab55210) | Abcam
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Recombinant Human Apolipoprotein E
Product name
Recombinant Human Apolipoprotein E
Protein length
Full length protein
Recombinant
Escherichia coli
Amino Acid Sequence
MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSE QVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET R ARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVR LAS HLRKLRKRLL RDADDLQKCL AVYQAGAREG AERGLSAIRE RL GPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRD RL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDM QRQWAGL VEKVQAAVGT SAAPVPSDNH
Molecular weight
Related Products
covers the use of
in the following tested applications.
The application notes include recommende optimal dilutions/concentrations should be determined by the end user.
Applications
Western blot
% SDS-PAGE.Assessed by SDS-PAGE and HPLC.
Endotoxin level: < 0.1 ng per ug of ApoE2
Lyophilised
Additional notes
This product is for the isoform APOE2
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 20mM Sodium phosphate, 0.5mM DTT, pH 7.8
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 20 mM Sodium Phosphate, pH 7.8 + 0.5 mM DTT to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer (e.g. PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Alternative names
Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Tissue specificity
Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Involvement in disease
Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians.
Sequence similarities
Belongs to the apolipoprotein A1/A4/E family.
Post-translationalmodifications
Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Western blot - Recombinant Human Apolipoprotein E
This product has been referenced in:
Robert J &et al. Clearance of beta-amyloid is facilitated by apolipoprotein E and circulating high-density lipoproteins in bioengineered human vessels. Elife 6:N/A (2017).
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Please note: All products are &FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE&
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PeproTech重组人ApoE4 1000ug
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PeproTech(派普泰克)
Recombinant Human ApoE4
Synonyms :
Apolipoprotein E4
Description :
ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE exists in
E2, E3, and E4, which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer’s disease. Recombinant human ApoE4 is a 34.4 kDa protein containing 300 amino acid residues.
Catalog #:
Greater than 90% by SDS-PAGE gel and HPLC analyses.
Endotoxin Level :
Endotoxin level is less than 0.1 ng per μg(1EU/μg).
Biological Activity :
Data Not Available.
AA Sequence :
MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVRGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH
PubMed Link :
Country of Origin :
Special Sizes:
Many products can be ordered in custom sizes. Please
to contact with your request.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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