Involvement of 4F2 antigen expressed on the MHC-negative target cells in the recognition of murine CD3+ CD4- CD8- αβ (Vα4/Vβ2)
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Department of Immunology and Cell Biology, Faculty of Medicine, Kyoto University Kyoto 606, Japan
1Torey Basic Research Institute Kamakura 248, Japan
2Tokyo Institute for Immunopharmacology Tokyo 171, Japan
Correspondence to: N. Minato
Received April
Accepted May
T cells of an unique phenotype (CD3+CD4-CD8- αβ TCR+) develop in vitro from the hematopoietlc progenitors, the majority of which carry homologous αβ TCR (Vα4Jα28/Vβ2Dβ1.1Jb.β2.6). In the present study, antigen corresponding to the particular αβ TCR was investigated, taking advantage of the
fact that growth of T cell hybridomas was arrested by their TCR stimulation. Results indicated that both syngenelc and allogeneic
thymocytes, particularly from newborn mice, could specifically inhibit the proliferation of a T cell hybridoma with the Vα4/Vβ2
TCR (15H1.2). Proliferation of neither TCR-missing hybridoma subciones nor those with unrelated αβ TCRs was affected at all.
It was also found that embryonal carcinoma (EC) cells without classical MHC antigens could specifically inhibit the proliferation
of 15H1.2 cells. We then raised a mAb, 14.37, against an EC line, OTF9, that could interfere with the ability of them to inhibit
the growth of 15H1.2. Pretreatment of 15H1.2 cells with anti-V&b.β2 and OTF9 cells with 14.37 mAb respectively completely
abrogated the growth inhibition of 15H1.2 by OTF9. The 14.37 antigen was a 120 kDa heterodimer glycoprotein consisting of
85 and 36 kDa proteins. In normal lymphold tissues, the expression of 14.37 antigen exhibited an apparently inverse relationship
with that of class I MHC antigens. Thus, ontogenlcally it was strongly expressed in fetal and newborn mice, rather rapidly
declined thereafter, and remained at very low levels in adult organs. In a given stage, the distribution of 14.37 antigen
and class I MHC was rather exclusive to each other. Molecular cloning of the 14.37 antigen revealed it to be a murine homolog
of human 4F2 heavy chain. Collectively, the present results suggested that a subset of CD3+ CD4- CD8- αβ T cells could specifically recognize the fetal/activation antigen, 4F2, on the target cells in a MHC-independent fashion.
Int. Immunol.
10.1093/intimm/6.9.1323
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