NIH3T3 Origin
NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 105 cells.” Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. George Todaro and Howard Green, the scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard
cell line.
NIH3T3 Characteristics
As one of the most commonly utilized cell lines, the NIH3T3 cell line has been incorporated in studies for a range of mechanistic and cell based assays, including protein functional analysis. The morphology of the cells are adherent, fibroblastic and are considered to be among the relatively easy to grow cell lines.
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The original cells are extremely contact inhibited, although the cell line is no longer inhibited. NIH 3T3 cells are receptive to transformation with polyomavirus and SV40. Also, 3T3 cells are sensitive to sarcoma virus focus formation, leukemia virus and are inhibited by temazepam and other benzodiazepines.
The NIH 3T3 cells doubling time is 20-26 hours.
Cell lines have a set of conditions for which they grow best. This includes a specific serum to provide additional growth factors, growth media, antibiotics to prevent bacterial growth and CO2 at a percentage for which the cells thrive (i.e. 5% CO2). Some cell lines require supplements such as non-essential amino acids (NEAA) or glutamine. Mammalian cells are grown in incubators set to 37°C.
Expression
Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway.
NIH3T3 Cytogenetics
3T3 mouse cells are hypertriploid. The modal chromosome number is 68, which occurs in 30% of cells. Higher ploidies occur at a much lower rate of 2.4%.
NIH 3T3 Cell Migration
Cell interactions with a surface influences behaviors such as proliferation, migration and differentiation. In order to study the behavior of a cell in relation to cell migration, the scrape or wound healing assay can be incorporated. Although there are many variations of the assay, the classic scrape assay is performed by making a scratch or wound in the monolayer of cultured cells. Using photographic data, cell migration into the area of the wound can be quantified using images captured during the experiment.
The scrape assay is an older technology with many flaws incorporated during the formation of the wound (i.e. inconsistent pressure applied to well bottom, cell confluency at wound site, etc). To combat these inconsistencies, there are now chemical and automated ways to create the wound for migration studies. Also, there are cartridge style assays in which cells exhibit migration through a membrane in pursuit of fresh culture medium.
Commercially available NIH/3T3 transfection reagent is available from
Research Articles
o Cytogenic characterization of the cell line: In this study, scientists studied the genome of NIH 3T3 cells. They characterized the gains and losses of copy numbers in NIH 3T3, and found that it had only gained four derivative chromosomes since its initial cytogenic study in 1989. Efforts to align NIH 3T3’s genome imbalances with homologous regions in humans indicated that imbalances in NIH 3T3 were similar to those in human solid cancers of the ectoderm.
o Comparison of NIH 3T3 cells to primary embryo cells: In an early study, the NIH 3T3 cells were compared to both other 3T3 cells and primary embryo cells. It was found that sensitivity to sarcoma virus formation and leukemia virus growth were comparable between NIH 3T3 cells and the other cells studied.
o Identification of Canine Distemper Virus (CDV) receptors for uptake: To determine what receptors CDV utilizes for uptake into susceptible cells, a monoclonal antibody was identified that binds to the cell surface and inhibits CDV infection. Once it was identified that CD9 was the receptor bound by this antibody, a plasmid was transfected into NIH 3T3 cells to express CD9. These cells were subsequently determined to be more permissive to CDV, however immunoprecipitation and virus-overlay protein binding assays were unable to determine if CDV is directly binding to the CD9 surface receptor.
o Mesothelioma glycoprotein antigen production: In this study, a cDNA fragment that encodes a glycoprotein common to mesothelial cells, mesothelioma and ovarian cancer cells was identified. The cDNA was then transfected into NIH 3T3 cells, which produced the glycoprotein on their surface. The glycoprotein was subsequently named mesothelin and may play a role in cellular adhesion.
o Reversible immortalization: NIH 3T3 cells were used as a control in a study of reversible immortalization of mammalian cells using an oncogene. Scientists were able to determine that the oncogene provided immortalization, and then, once excised, cells reverted to their pre-immortalization characteristics.NIH3T3（人胚肾细胞）_细胞株-上海抚生试剂公司
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NIH3T3（人胚肾细胞） 细胞规格：1.2*10&5细胞形态： 细胞株 生长特性：贴壁 悬浮 半贴壁传代时间：2-3天 3-5天 传代比例：1:2~1:3& 1:1~1:2培养条件：DMEM+10%/胎牛血清，37℃，5%CO2冻存条件：常规培养基，20%FBS，10%DMSO&NIH3T3（人胚肾细胞） 传代方法：细胞完全汇合时，倒掉旧液，加入消化液（0.25%胰蛋白酶+0.03%EDTA）2ml消化，显微镜下观察细胞完全脱离瓶壁分离成单个细胞后弃掉胰酶，加培养基混匀分瓶，T25瓶中加培养基至6-8ml，T75瓶加培养基至20ml，37℃，5%CO2孵箱培养。邮购备注： 收到细胞后，请镜下观察细胞，用恰当方式处理细胞。若有悬浮的细胞，请离心收集细胞，接种到一个新的培养瓶中。使用新鲜配制的培养基，使用进口胎牛血清，刚接到细胞时血清浓度可以在15％。公司其他细胞产品：
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NIH3T3细胞 NIH3T3细胞
　　做细胞实验的同学看过来。我司是您科研路上的好帮手！　　我司提供NIH3T3细胞，经营多种&ATCC细胞株,ScienCell细胞，菌株，细胞系,动植物细胞,标准菌株&，竭诚为您提供的完善的售前、售中和售后服务，专业提供&NIH3T3细胞&技术咨询等服务。　　选择上海斯信生物NIH3T3细胞有3个理由：　　1、细胞状态好，形态佳，易成活，是市面上比较好养的细胞之一;　　2、物流迅速，复苏好后发货，次日就能到;　　3、使用我司推荐的进口品牌血清进行培养实验，效果更佳。&　　NIH3T3细胞及部分细胞株简介:　　细胞简称 35.1 中文名称 杂交瘤细胞抗CD2 培养条件　　细胞简称 5637 中文名称 人膀胱癌细胞 培养条件 1640　　细胞简称 MHCC97-L 中文名称 低转移人肝癌细胞 培养条件 DMEM　　细胞简称 U-2 OS 中文名称 人骨肉瘤细胞 培养条件 DMEM　　细胞简称 16HBE 中文名称 人支气管上皮细胞 培养条件 1640　　细胞简称 22RV1 中文名称 人前列腺癌细胞 培养条件　　细胞简称 293 [HEK-293] 中文名称 人胚肾细胞 培养条件　　细胞简称 293 Cells, low passage 中文名称 人胚肾细胞 培养条件　　细胞简称 293ebna 中文名称 人胚肾细胞 培养条件　　细胞简称 293FT 中文名称 人胚肾细胞 培养条件　　细胞简称 293-T 中文名称 人胚肾细胞 培养条件 DMEM　　细胞简称 2T3 中文名称 小鼠成骨细胞 培养条件　　细胞简称 2V6.11 中文名称 人胚肾细胞 培养条件　　细胞简称 3T3-L1 中文名称 小鼠胚胎成纤维细胞 培养条件 DMEM 10%　　细胞简称 3T3-Swiss 中文名称 鼠胚胎成纤维细胞 培养条件 DMEM 10%　　细胞简称 3T6-Swiss albino 中文名称 小鼠胚胎成纤维细胞 培养条件　　细胞简称 4T1 中文名称 小鼠腺癌细胞 培养条件 1640 10%FBS　　细胞简称 5-8F 中文名称 人鼻咽癌细胞 培养条件　　细胞简称 6-10B 中文名称 人鼻咽癌细胞 培养条件　　细胞简称 6T-CEM 中文名称 人T细胞白血病细胞 培养条件　　细胞简称 769-P 中文名称 人肾细胞腺癌细胞 培养条件　　细胞简称 786-0 中文名称 人肾透明细胞腺癌细胞 培养条件 1640 10%FBS　　细胞简称 95-C 中文名称 人低转移肺癌细胞 培养条件 DMEM　　细胞简称 9-5D 中文名称 人高转移肺腺癌细胞 培养条件　　细胞简称 95-D 中文名称 人高转移肺癌 培养条件 1640 10%FBS　　细胞简称 A172 中文名称 人胶质母细胞瘤细胞 培养条件　　细胞简称 A2780 中文名称 人卵巢癌细胞 培养条件　　细胞简称 A3 中文名称 人T淋巴细胞白血病细胞 培养条件 1640　　细胞简称 A-375 中文名称 人恶性黑色素瘤细胞 培养条件 DMEM　　细胞简称 A-431 中文名称 人表皮癌细胞 培养条件 DMEM　　细胞简称 A498 中文名称 人肾癌细胞 培养条件　　细胞简称 A549 中文名称 人肺癌细胞 培养条件 1640 10%FBS　　细胞简称 A-673 中文名称 人横纹肌瘤细胞 培养条件　　细胞简称 A7r5 中文名称 大鼠胸大动脉平滑肌细胞 培养条件 DMEM　　细胞简称 A9 中文名称 小鼠皮下结缔组织细胞 -tx-培养条件&
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