A protein interaction map of Drosophila melanogaster. - Abstract - Europe PMC
Europe PMC requires Javascript to function effectively.
Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please
turn on Javascript support in your web browser and reload this page.
http://orcid.org
Search worldwide, life-sciences literature
Joel Bader
Chaudhuri A
Vijayadamodar G
Machineni H
Yale University
Zerhusen B
McDaniel L
Stimpson E
Williams J
Renzulli R
Aanensen N
Carrolla S
Bickelhaupt E
Lazovatsky Y
Clement Stanyon
Finley RL Jr
Braverman M
Shimkets RA
McKenna MP
Rothberg JM
[06 Nov 51):]
Research Support, U.S. Gov't, P.H.S., Journal Article
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.
)- subscription required
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
Show all items
CitePeer Related Articles}